Description
This ELISA kit is a stable and convenient assay system for peptide YY (PYY). PYY was isolated initially by Tatemoto et al. (1980 ) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acid residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum) showing an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level decreases after resection of the intestine possibly are due to the decrease in number of the endocrine cells secreting PYY. The EIA kit is prepared by using synthetic mouse/rat PYY (3-36) as standard antigen and biotinylated mouse/rat PYY (3-36) as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence of Mouse/rat PYY. The mouse/rat PYY sequence:
Y-P-A-K-P-E-A-P-G-E-D-A-S-P-E-E-L-S-R-Y-Y-A-S-L-R-H-Y-L-N-L-V-T-R-Q-R-Y-NH2